University of agribusiness and rural development, UARD Jubilee International Scientific Conference

Font Size: 
Ex situ (in field) conservation of Bulgarian species from genus Mentha maintained in the collection of IPGR-Sadovo
Katya Uzundzhalieva, Stanislava Stateva

Last modified: 2018-02-12

Abstract


The genus Mentha belongs to the botanical family Lamiaceae. In Bulgaria are spread 6 varieties and numerous natural hybrids. The species from the genus Mentha are been well known since ancient days as oil and honey bearing plants. That is why it is considered that most of the known nowadays species are very old. In the Institute of Plant Genetic Resources – Sadovo are maintained in field two Mentha varieties. M. arvensis L and M. spicata L. Besides the in field conservation of the species, in vitro techniques are a reliable means of reproduction and long-term storage. In the experimental work for in vitro conservation of microcuttings of M. arvensis L and M. spicata L in low temperatures were applied 4 different temperatures - 2ºС, 4ºС, 6ºС and 8ºС. Longest conservation period (12 months) with highest level of survived cuttings (83,50%) was observed in 4ºС. After cultivation of the newly obtained microcuttings from in vitro conservation in normal conditions (24ºС) were observed little differences between two mint varieties  in shoot growth, which is due to their specific biology and the period of in vitro conservation under 4ºС has no influence

Keywords


genus Mentha, M. arvensis L., M. spicata L., in field conservation, in vitro conservation, Lamiaceae.

References


  1. Chishiti, N., A. Shawl, Z. Kaloo, M. Bhat, P. Sultan. 2006. Clonal propagation of Mentha arvensis L. through nodal explant. Pakistan journal of biological science, 9, 8 , 1416-1419.
  2. Desheva, Gergana., 2009. Conservation of wheat germplasm (Triticum aestivum L. and Triticum durum Desf.) identity in genebank.Disertation, Sadovo, 2009.
  3. Engelmann. F. 2011. Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular and Developmental Biology - Plant, Vol.  47, 5-16.
  4. 4.. Gonçalves, S, A. Romano. 2007. In vitro minimum growth for conservation of Drosophyllum lusitanicum. Biologia Plantarum, Vol. 51, 795-798
  5. 5.Grenan, S. 1979. Rhizogenese du bourgeons apicaux de boutures de vigne cultivees in vitro. Vigne Vin, 13, 125-130.
  6. Islam, T., S. Leunufna, D, Dembele, E. keller. 2003. In vitro conservation of four mint /Mentha spp./ accessions. Plant tissue culture, 13, 1, 37-46
  7. Lata, H., R. Moraes, B. Bertoni, A. Pereira. 2010. In vitro germplasm conservation of Podophyllum peltatum L. under slow growth conditions. In Vitro Cellular and Developmental Biology-Plant, Vol. 46, 22-27.
  8. Reed, B. 1999. In vitro storage conditions of mint germplasm. Hortscience, 34/2/, 350-352.
  9. Selvakumar, V.,  R. Anbudural, T. Balakumar. 2001. In vitro propagation of the M. piperita L. through nodal explants. In Vitro Cell. Dev. Biol. Plant., 37, 280-284.
  10. 10.Shibli. R., M. Shat nowi, W. Subaih, M.Ajlouni. 2006. In vitro conservation and cryopreservation of plant genetic resources: Review. World journal Agricultural science, 2/4/ 372-382.
  11. Velchev, V., 1989. Flora of People`s Republic of Bulgaria. Vol. IX.
  12. Withers, L., J. Engels. 1990. The test tube genebank - a safe alternative to field conservation. IBPGR Newsletter for Asia and the Pacific, 3, 1-2.

 


Full Text: PDF